Laboratory of Subcellular Structures Analyses

Prof. Eliza Wyszko

Head of Laboratory
eliza.wyszko@ibch.poznan.pl
ext. 1141

  • low molecular compounds,
  • nucleosides,
  • DNA modifications,
  • catalytic nucleic acids,
  • gene expression,
  • confocal microscopy,
  • flow cytometry,
  • real time PCR,
  • xCELLigence,
  • CO2 incubator,
  • laminar flow cabinet,
  • high-performance liquid chromatography,
  • HPLC,
  • MS

Keywords

  • Confocal microscope Leica TCS SP5 II with white laser and intravital chamber with LAS AF SP5 software and LAS X SP8 software with deconvolution

  • DMI 4000B Leica microscope: fluorescence, bright field

  • Stereoscopic microscope Leica

  • High-performance liquid chromatography (HPLC) with detectors: UV, fluorescent (FLD), electrochemical (ED) and mass (MSI)

  • FACSCalibur flow cytometer (Becton Dickinson)

  • Accuri C6 flow cytometer (Becton Dickinson)

  • Cell harvester (96 wells) Perkin Elmer

  • Real time PCR LightCycler 480 II Roche

  • xCELLignce system

  • Cell culture CO2 incubator INCO 153 Memmert

  • Laminar flow cabinet BioBan 48 for sterile cell culturing

Equipment

  • Skupińska M, Stępniak P, Łętowska I, Rychlewski L, Barciszewska M, Barciszewski J,Giel-Pietraszuk M.Natural Compounds as Inhibitors of Tyrosyl-tRNA SynthetaseMicrob Drug Resist. 23:308-320 (2017)
  • Orlicka-Płocka M, Gurda D, Fedoruk-Wyszomirska A, Smolarek I, Wyszko E.Circulating microRNAs in Cardiovascular DiseasesActa Biochim Pol. 63:725-729 (2016)
  • Belter A, Rolle K, Piwecka M, Fedoruk-Wyszomirska A, Naskręt-Barciszewska MZ, Barciszewski J.Inhibition of miR-21 in glioma cells using catalytic nucleic acidsSci Rep. 15:24516 (2016)
  • Gabryelska MM, Wyszko E, Barciszewski J.RNA dactyloscopyActa Biochim Pol. 3:785-787 (2016)
  • Fedoruk-Wyszomirska A, Markiewicz WT, Barciszewski J.New inhibitors of DNA methyltransferase 1 based on cytosine scaffoldBioTechnologia 97:323-325 C (2016)
  • Fedoruk-Wyszomirska A, Szymański M, Głodowicz P, Gabryelska M, Wyszko E,Estrin WJ, Barciszewski J.Inhibition of HIV-1 gp41 expression with hammerhead ribozymesBiochem J. 471:53-66 (2015)
  • Kucinska M, Skupin-Mrugalska P, Szczolko W, Sobotta L, Sciepura M, Tykarska E, Wierzchowski M, Teubert A, Fedoruk-Wyszomirska A, Wyszko E, Gdaniec M, Kaczmarek M, Goslinski T, Mielcarek J, Murias M.Phthalocyanine derivatives possessing 2-(morpholin-4-yl)ethoxy groups as potential agents for photodynamic therapyJ Med Chem. 58:2240-2255 (2015)
  • Mileshina D, Niazi AK, Wyszko E, Szymanski M, Val R, Valentin C, Barciszewski J, Dietrich A.Mitochondrial targeting of catalytic RNAs. MethodsMol Biol. 1265:227-254 (2015)
  • Barciszewska AM, Gurda D, Głodowicz P, Nowak S, Naskręt-Barciszewska MZ.A New Epigenetic Mechanism of Temozolomide Action in Glioma CellsPLoS One. 2015, 10: e0136669
  • Gurda D, Handschuh L, Kotkowiak W, Jakubowski H.Homocysteine thiolactone and N-homocysteinylated protein induce pro-atherogenic changes in gene expression in human vascular endothelial cellsAmino Acids. 47:1319-1339 (2015)
 

Selected publications

  • Kinetin riboside and its derivatives - analysis of apoptotic properties and mechanism of action in brain tumor cells. NSC – OPUS 7

  • Anti-aging properties of 4-N-furfyrylocytosine in age-differentiated eukaryotic cells, budding yeast and mouse model of aging. NSC – OPUS 13

  • The regulation of neuroactive beta-carboline levels in diet based on the coffee substitute. NSC – OPUS 10 (cooperation with Poznań University of Life Sciences)

Research activity

Confocal microscopy:

  • Visualization of intravital and fixed preparations, 
  • Analysis of fluorochromes, ex. 470-670 nm and 405 nm, 
  • 2D and 3D analysis of images, 
  • FRET, FRAP,
  • Analysis of fluorescence intensity,
  • Colocalization, 

Flow cytometry:

  • Analysis of cell cycle,
  • Analysis of cell toxicity (apoptosis, necrosis), 
  • Immunofluorescent staining,
  • Analysis of oxidative stress,
  • Cell sorting, 

xCELLigence system:

  • Real time analysis of cell proliferation or migration,

Termocycler LightCycler 480:

  • Real time PCR with fluorescent probes,
  • HRM analysis of point mutations,
  • Analysis of methylation level and genotyping,

High-performance liquid chromatography (HPLC):

  • Analysis of nucleic acid modifications in eukaryotic cells. 
     

Possibility of carrying out the entire experimental process including cell culturing and the use of specialized equipment by PASS.
 

Offer

Research Projects

Dr. habil. Małgorzata Giel-Pietraszuk

profesor instytutu

Researchers:

Dr. Dorota Gurda-Woźna

starszy specjalista biolog

Dr. Agnieszka Fedoruk-Wyszomirska

starszy specjalista biolog

Technical Staff:

Marta Orlicka-Płocka MSc, BEng.

PhD student

Paweł Pawelczak MSc

PhD student

PhD students:

Media Społecznościowe

Strona zrobiona w kreatorze stron internetowych WebWave

Cooperation

Institute of Bioorganic Chemistry,
Polish Academy of Sciences
Z. Noskowskiego 12/14
61-704 Poznań
tel centrala: (+48) 61 852 85 03
fax: (+48) 61 852 05 32
e-mail: ibch@ibch.poznan.pl

Sekretariat Dyrektora, tel: 61 852 89 19

Adress