Laboratory of Genomics

Dr. Luiza Handschuh

Head of Laboratory
luizahan@ibch.poznan.pl
Phone: 61 6653052

  • Handschuh L, Kaźmierczak M, Milewski MC, Góralski M, Łuczak M, Wojtaszewska M, Uszczyńska-Ratajczak B, Lewandowski K, Komarnicki M, Figlerowicz M.Gene expression profiling of acute myeloid leukemia samples from adult patients with AML-M1 and -M2 through boutique microarrays, real-time PCR and droplet digital PCRInt J Oncol. doi: 10.3892/ijo.2017.4233 (2017)

  • Samelak-Czajka A, Marszalek-Zenczak M, Marcinkowska-Swojak M, Kozlowski P, Figlerowicz M, Zmienko A.MLPA-Based Analysis of Copy Number Variation in Plant PopulationsFront Plant Sci. 8:222 (2017)

  • Marcinkowska-Swojak M, Handschuh L, Wojciechowski P, Goralski M, Tomaszewski K, Kazmierczak M, Lewandowski K, Komarnicki M, Blazewicz J, Figlerowicz M, Kozlowski P. Simultaneous detection of mutations and copy number variation of NPM1 in the acute myeloid leukemia using multiplex ligation-dependent probe amplificationMutation Research 786:14-26 (2016)

  • Klonowska K, Handschuh L, Swiercz A, Figlerowicz M, Kozlowski P.MTTE: an innovative strategy for the evaluation of targeted/exome enrichment efficiencyOncotarget. 7:67266-67276 (2016)

  • Zmienko A, Samelak-Czajka A, Kozlowski P, Szymanska M, Figlerowicz M.Arabidopsis thaliana population analysis reveals high plasticity of the genomic region spanning MSH2, AT3G18530 and AT3G18535 genes and provides evidence forNAHR-driven recurrent CNV events occurring in this locationBMC Genomics, 17(1):893 (2016)

  • Goralski M., Sobieszczanska P., Obrepalska-Steplowska A., Swiercz A., Zmienko A., Figlerowicz M.A gene expression microarray for Nicotiana benthamiana based on de novo transcriptome sequence assemblyPlant Methods 12:28 (2016)

  • Zmienko A, Goralski M, Samelak-Czajka A, Sobieszczuk-Nowicka E, Figlerowicz M.Time course transcriptional profiling of senescing barley leavesGenom Data, , 4:78-81 (2015)

  • Zmienko A, Samelak-Czajka A, Goralski M, Sobieszczuk-Nowicka E, Kozlowski P, Figlerowicz M.Selection of reference genes for qPCR- and ddPCR-based analyses of gene expression in Senescing Barley leavesPLoS One. 10(2):e0118226 (2015)

  • Zyprych-Walczak J, Szabelska A, Handschuh L, Górczak K, Klamecka K, Figlerowicz M, Siatkowski I.The Impact of Normalization Methods on RNA-Seq Data AnalysisBiomed Res Int. 2015:621690 (2015)

 

Selected publications

Copy number variation of Arabidopsis thaliana MSH2 gene - the structure of duplication, range of polymorphism in natural ecotypes and its impact on the expression of genes involved in DNA mismatch repair pathway. NCN – Miniatura 

Research activity

  • QX200 Droplet Digital PCR (ddPCR™) System (BioRad)

  • Rotor-Gene Q real-time PCR cycler (Qiagen)

  • 2100 Bioanalyzer (Agilent)

  • NanoPrint LM60 microarray printer (Telechem)

  • SpotArray24 microarray printer (PerkinElmer)

  • HS 4800 Pro microarray hybridization station (Tecan)

  • Axon 4200AL microarray scanner (Molecular Devices)

  • ScanArray Express microarray scanner (PerkinElmer)

  • DNA CL E508 G Crosslinker (Uvitec)

  • Bioruptor NextGen sonication system (Diagenode)

  • Nanodrop 2000 spectrophotometer (Thermo Scientific)

  • Qubit Fluorometer (Invitrogen)

  • Thermocyclers (Techne, BioRad)

  • Quantum St4 system for gel documentation (Fisher Biotec)

Equipment

Based on the development facilities and many years of experience, we can offer support in genomic research, from microarrays through next generation sequencing (DNA- and RNA-seq) to quantitative PCR-based analysis of selected genes/transcripts.

Offer

  • genomics,

  • next generation sequencing,

  • microarrays,

  • gene expression analysis,

  • computational biology 

Keywords

Research Projects

Dr. Paweł Wojciechowski

adiunkt

Researchers:

Anastasiia Satyr MSc, BEng.

PhD student

Dr. habil. Aleksandra Świercz

starszy specjalista biolog

Dr. Jan Podkowińśki

starszy specjalista biolog

Dr.Agnieszka Żmieńko

starszy specjalista biolog

Dr. Anna Samelak-Czajka

biologist

Magdalena Rakoczy MSc

 biologist

Technical Staff:

Marek Milewski MCs

biologist

Aleksander Strugała MSc. BEng.

biologist

PhD students:

Media Społecznościowe

Strona zrobiona w kreatorze stron internetowych WebWave

Cooperation

Institute of Bioorganic Chemistry,
Polish Academy of Sciences
Z. Noskowskiego 12/14
61-704 Poznań
tel centrala: (+48) 61 852 85 03
fax: (+48) 61 852 05 32
e-mail: ibch@ibch.poznan.pl

Sekretariat Dyrektora, tel: 61 852 89 19

Adress