Institute of Bioorganic Chemistry,
Polish Academy of Sciences
Z. Noskowskiego 12/14
tel centrala: (+48) 61 852 85 03
fax: (+48) 61 852 05 32
Sekretariat Dyrektora, tel: 61 852 89 19
Fritzell K, Xu LD, Otrocka M, Andréasson C, Öhman M. Sensitive ADAR editing reporter in cancer cells enables high-throughput screening of small molecule libraries. Nucleic acids research 2019 47;1-11 https://doi.org/10.1093/nar/gky1228
Tenland E, Krishnan N, Rönnholm A, Kalsum S, Puthia M, Mörgelin M, Davoudi M, Otrocka M, Alaridah N, Glegola-Madejska I, Sturegård E, Schmidtchen A, Lerm M, Robertson BD, Godaly G. A novel derivative of the fungal antimicrobial peptide plectasin is active against Mycobacterium tuberculosis. Tuberculosis (Edinburgh, Scotland) 2018 113; 231-238
Axelsson H, Almqvist H, Otrocka M, Vallin M, Lundqvist S, Hansson P, Karlsson U, Lundbäck T, Seashore-Ludlow B. In Situ Target Engagement Studies in Adherent Cells. ACS chemical biology 2018 13;4 942-950 https://doi.org/10.1021/acschembio.7b01079
Pettersson-Klein AT, Izadi M, Ferreira DMS, Cervenka I, Correia JC, Martinez-Redondo V, Southern M, Cameron M, Kamenecka T, Agudelo LZ, Porsmyr-Palmertz M, Martens U, Lundgren B, Otrocka M, Jenmalm-Jensen A, Griffin PR, Ruas JL. Small molecule PGC-1α1 protein stabilizers induce adipocyte Ucp1 expression and uncoupled mitochondrial respiration. Molecular metabolism 2018 9; 28-42 https://doi.org/10.1016/j.molmet.2018.01.017
Dyczynski M, Yu Y, Otrocka M, Parpal S, Braga T, Henley AB, Zazzi H, Lerner M, Wennerberg K, Viklund J, Martinsson J, Grandér D, De Milito A, Pokrovskaja Tamm K. Targeting autophagy by small molecule inhibitors of vacuolar protein sorting 34 (Vps34) improves the sensitivity of breast cancer cells to Sunitinib. Cancer letters 2018 435; 32-43 https://doi.org/10.1016/j.canlet.2018.07.028
Pinheiro T, Otrocka M, Seashore-Ludlow B, Rraklli V, Holmberg J, Forsberg-Nilsson K, Simon A, Kirkham M. A chemical screen identifies trifluoperazine as an inhibitor of glioblastoma growth. Biochemical and biophysical research communications 2017 494;3-4 477-483
„TMPRSS2- potencjalny cel dla nowych leków oraz wyznacznik przebiegu COVID19- NCN Szybka ścieżka przebiegu COVID19”, Dr. Paweł Zmora, IBCH/PAN
“Inhibition of biocalcification” – EU-OPENSCREEN DRVIE, Prof. Ingvar Jarle Vaage, University of w Oslo
Shared at CHTSS
Opera Phenix (Perkin Elmer) High Content Screening System
CLARIOstar Plus (BMGLabtech) multimode plate reader
Bravo Pipetting station (Agilent)
Tempest liquid handlers (Formulatrix)
Robotic arm (Mitsubishi)
The aim of Laboratory of Molecular Assays is to support scientists in transforming their research into projects allowing identification of small molecules that can serve as chemical tools or starting points for further development into new medicines or other industrial use.
The Laboratory is a part of Centre of High Throughput Screening Studies (CHTSS), located in the Centre of Advanced Technologies and is offering know-how and hands on help in development of industrial standard screening assays and performing small molecule screens. Our experience is covering target based biochemical techniques as well as cell-based assays, including phenotyping high content screening and strong expertise in image analysis.
We collaborate closely with Laboratory of Hight Throughput Screening and together form one of EU-OPENSCREEN (https://www.eu-openscreen.eu) screening site - the only laboratory in Poland with the capability of performing high throughput screening campaigns. With an access to EU-OPENSCREEN chemical library and state of art equipment, we can test biological activity of hundred thousand molecules within days including additional chemical libraries if required.
We welcome all the researchers from different disciplines, academia as well as industry, who want to miniaturise and optimise their bench-top model and use in testing against a unique compound collection. We will assist you in establishing conditions for running your assay in 96, 384 or 1536 well plates, finding necessary controls and, finally, apply liquid handling and robotics, to ensure that the results of your screening campaign are reliable. We perform primary screening, orthogonal tests, analyse the data and eventually confirm activity providing IC50 values for selected molecules. Hit compounds can be also tested for their toxicity in different cellular models.
High Throughput Screening,
High Content Screening,
Target Based Screening,