Department of Medical Biotechnology

Dr. habil. Agnieszka Fiszer

Head of Department:
agnieszka.fiszer@ibch.poznan.pl
ext. 1135

Pracownicy naukowi

Dr. Magdalena Jazurek-Ciesiołka

adiunkt

Dr. Grzegorz Figura

asystent

Dr. Emilia Kozłowska

adiunkt

Dr. Adam Ciesiołka

adiunkt

Dr. Magdalena Woźna -Wysocka

adiunkt

Dr. Paweł Świtoński

asystent

Paula Sobieszczańska MSc

specjalista biolog

Technical Staff:

Agata Ciołak MSc

doktorant

Paweł Joachimiak MSc

doktorant

PhD students:

Researchers:

  • Ciesiolka A, Stroynowska-Czerwinska A, Joachimiak P, Ciolak A, Kozlowska E, Michalak M, Dabrowska M, Olejniczak M, Raczynska KD, Zielinska D, Wozna-Wysocka M, Krzyzosiak WJ, Fiszer A. Artificial miRNAs targeting CAG repeat expansion in ORFs cause rapid deadenylation and translation inhibition of mutant transcripts. Cell Mol Life Sci. 2020 Jul 21. doi: 10.1007/s00018-020-03596-7

  • Nowak B, Fiszer A. Strategie wyciszania ekspresji zmutowanego genu w terapii choroby Huntingtona. Postępy Biochemii 2020 Tom 66 Nr 1

  • Ciolak A, Krzyzosiak WJ, Kozlowska E, Fiszer A. Generation of human iPS cell line IBCHi002-A from spinocerebellar ataxia type 3/Machado-Joseph disease patient's fibroblasts. Stem Cell Res. 2020 Apr 20;45:101796. doi: 10.1016/j.scr.2020.101796.

  • Dabrowska M, Ciolak A, Kozlowska E, Fiszer A, Olejniczak M. Generation of New Isogenic Models of Huntington's Disease Using CRISPR-Cas9 Technology. Int J Mol Sci. 2020 Mar 8;21(5). pii: E1854. doi: 10.3390/ijms21051854.

  • Misiorek JO, Schreiber AM, Urbanek-Trzeciak MO, Jazurek-Ciesiołka M, Hauser LA, Lynch DR, Napierala JS, Napierala M. A Comprehensive Transcriptome Analysis Identifies FXN and BDNF as Novel Targets of miRNAs in Friedreich's Ataxia Patients. Mol Neurobiol. 2020 Apr 14. doi: 10.1007/s12035-020-01899-1

  • Stoyas CA, Bushart DD, Switonski PM, Ward JM, Alaghatta A, Tang M, Niu C, Wadhwa M, Huang H, Savchenko A, Gariani K, Xie F, Delaney JR, Gaasterland T, Auwerx J, Shakkottai VG, La Spada AR. Nicotinamide Pathway-Dependent Sirt1 Activation Restores Calcium Homeostasis to Achieve Neuroprotection in Spinocerebellar Ataxia Type 7. Neuron. 105(4):630-644.e9 (2020)

  • Kozlowska E, Ciolak A, Olejniczak M, Fiszer A. Generation of human iPS cell line IBCHi001-A from dentatorubral-pallidoluysian atrophy patient's fibroblasts Stem Cell Res. 39:101512 (2019)

  • Ward JM, Stoyas CA, Switonski PM, Ichou F, Fan W, Collins B, Wall CE, Adanyeguh I, Niu C, Sopher BL, Kinoshita C, Morrison RS, Durr A, Muotri AR, Evans RM, Mochel F, La Spada AR. Metabolic and Organelle Morphology Defects in Mice and Human Patients Define Spinocerebellar Ataxia Type 7 as a Mitochondrial Disease. Cell Rep. 26(5):1189-1202.e6 (2019)

  • Kulcenty K, Wroblewska JP, Rucinski M, Kozlowska E, Jopek K, Suchorska WM. MicroRNA Profiling During Neural Differentiation of Induced Pluripotent Stem Cells. Int J Mol Sci. 20(15): E3651 (2019)

  • Witkos T, Krzyzosiak WJ, Fiszer A, Koscianska E. A potential role of extended simple sequence repeats in competing endogenous RNA crosstalk. RNA Biol. 15(11):1399-1409 (2018)

Selected publications

  • Comprehensive analysis of the therapeutic potential of oligonucleotides for the treatment of polyglutamine diseases (NCN SONATA, PI: Agnieszka Fiszer)

  • Identification of CAG repeat-binding proteins and imaging RNA-protein interactions in cells (NCN OPUS, PI: Adam Ciesiolka)

  • RAN translation at CAG repeats in spinocerebellar ataxia type 3 (NCN SONATA, PI: Magdalena Jazurek-Ciesiołka).

  • MicroRNA profiling in cellular models of Huntington’s disease generated using iPSC technology (NCN PRELUDIUM, PI: Emilia Kozłowska)

  • Investigation of initial pathways of DRPLA pathogenesis in human neural precursors (MNiSW Diamentowy Grant, B. Nowak, Tutor: A. Fiszer)

  • Development and production of a diagnostic kit for the detection of the SARS-CoV-2 coronavirus causing COVID-19 (MNiSW, department members involved : A. Fiszer, A. Ciesiołka, G. Figura, P. Joachimiak, P. Sobieszczańska)

Research activity

  • triplet repeat expansion diseases,
  • Huntington’s disease,
  • pathogenesis,
  • RNA toxicity,
  • cellular models,
  • mouse models,
  • experimental treatment,
  • RNA interference technology,
  • iPSC technology,
  • microRNA biogenesis and function

Research performed in our Department has been focusing on trinucleotide repeats sequences and their expansion related to human neurological diseases. This group of diseases includes: myotonic dystrophy type 1 (DM1) and polyglutamine (polyQ) diseases: Huntington’s disease (HD) and several spinocerebellar ataxias (SCA).

Main research topics carried out in our Department are:

  • screening for RNA and protein toxicity markers in polyglutamine diseases,

  • establishment and characteristics of new cellular and mouse models,

  • investigation of proteins interaction with trinucleotide repeats tracts,

  • testing of therapeutic strategies targeting CAG repeats regions in neuronal cells and mouse models,

  • unravelling mechanisms responsible for siRNA and miRNA silencing of gene expression,

  • evaluation of the miRNA role in pathogenesis of polyglutamine diseases.

 

Research area

Keywords

Research Projects

Media Społecznościowe

Strona zrobiona w kreatorze stron internetowych WebWave

Cooperation

Institute of Bioorganic Chemistry,
Polish Academy of Sciences
Z. Noskowskiego 12/14
61-704 Poznań
tel centrala: (+48) 61 852 85 03
fax: (+48) 61 852 05 32
e-mail: ibch@ibch.poznan.pl

Sekretariat Dyrektora, tel: 61 852 89 19

Adress