Department of RNA Structural Genomics

Prof. Elżbieta Kierzek

Head of Department
elzbieta.kierzek@ibch.poznan.pl
ext. 1113, 1112

Barbara Szutkowska MSc

PhD Student

Aleksandra Jarmołowicz MSc

PhD Student

Klaudia Wieczorek MSc, BEng

PhD Student

PhD Students:

Aleksandra Jarmołowicz MSc

chemist

Technical Staff:

Dr. Elżbieta Lenartowicz
asystent

Dr. Julita Piasecka
asystent

Researchers:

  • RNA structural genomics,
  • RNA viruses,
  • influenza virus,
  • SARS-CoV-2,
  • COVID-19,
  • isoenergetic microarrays,
  • modified microarray probes,
  • nucleic acids thermodynamics of nucleic acids,
  • modified oligonucleotides,
  • antisense oligonucleotides,
  • siRNA,
  • small molecules,
  • RNA structure,
  • RNA interactions, pathogenic RNA
  • RNA structural genomics of viruses

  • Secondary and tertiary structure of RNA and its complexes with functional proteins, other RNA and small molecules

  • Modulation RNA functional activities, including pathogenic RNAs involved with human diseases 

  • RNA structural genomics of influenza virus, influence of RNA structure on virus replication

  • Study of structure and interactions of selected segments of influenza virus vRNA(-) and RNA(+) and important for function structural motifs of virus RNA in vitro and in vivo. 

  • Determination in the RNA of influenza virus the regions of modified oligonucleotides and ligands strong binding

  • Improving microarrays mapping method with isoenergetic microarrays 

  • Projecting and optimization of modified oligonucleotides, siRNA, ribozymes and ligands as potential inhibitors of influenza virus life cycle. 

  • Study of structures and interactions of complexes of influenza virus vRNA(-) and virus nucleoprotein (NP).

  • Inhibition and regulation of influenza virus proliferation – cells studies.

  • Modified oligonucleotides – antisense and microarrays strategies. 

  • Oligonucleotide carriers and bioconjugates.

  • CRISPR/Cas systems as strategies for replication inhibitors of RNA viruses.

  • Thermodynamic and comparison analysis of studied RNA, prediction of RNA secondary structure.

  • Studies of functional RNA structural motifs of SARS-CoV-2 virus

  • Modified oligonucleotides with inhibitory activities against SARS-CoV-2 virus proliferation

  • Searching for small molecules that inhibit SARS-CoV-2 virus proliferation

  • Cells tests of potential inhibitors of SARS-CoV-2 virus replication

  • Binding site and structural foundations for disruption of SARS-CoV-2 virus replication by inhibitors

Research area

Keywords

Research Projects

  • J. Piasecka, E. Lenartowicz, M. Soszynska-Jozwiak, B. Szutkowska, R. Kierzek, E. Kierzek,  RNA Secondary Structure Motifs of the Influenza A Virus as Targets for siRNA-Mediated RNA Interference. Molecular Therapy - Nucleic Acids, 19, 627–642 (2020).

  • J. Kesy, K.M. Patil, S.R. Kumar, Z.Y. Shu, H.Y. Yong, L. Zimmermann, A.A.L. Ong, D.F.K. Toh, M.S. Krishna, L.X. Yang, J.L. Decout, D.H. Luo, M. Prabakaran, G. Chen and E. Kierzek, A Short Chemically Modified dsRNA Binding PNA (dbPNA) Inhibits Influenza Viral Replication by Targeting Viral RNA Panhandle Structure. Bioconjugate Chemistry, 3, 931-943 (2019)

  • P. Michalak, M. Soszynska-Jozwiak, E. Biala, W.N. Moss, J. Kesy, B. Szutkowska, E. Lenartowicz, R. Kierzek, E. Kierzek, Secondary structure of the segment 5 genomic RNA of influenza A virus and its application for designing antisense oligonucleotides. Scientific Reports, 9, 3801 (2019)

  • M. Szabat, E. Kierzek, R. Kierzek, Modified RNA triplexes: Thermodynamics, structure and biological potential. Scientific Reports, 8, 13023 (2018)

  • M. Soszyńska-Jóźwiak, P. Michalak, W. N. Moss, R. Kierzek, J. Kesy, Elzbieta Kierzek, Influenza virus segment 5 (+)RNA - secondary structure and new targets for antiviral strategies. Scientific Reports, 7: 15041 (2017)

  • A Dembska, E. Kierzek, B. Juskowiak, Studying the influence of stem composition in pH-sensitive molecular beacons onto their sensing properties. Analytica Chimica Acta, 990, 157-167 (2017)

  • Ruszkowska, E. Lenartowicz, W. N. Moss, R. Kierzek and E. Kierzek, Secondary structure model of the naked segment 7 influenza A virus genomic RNA. Biochemical Journal, 473, 4327-4348 (2016).

  • E. Lenartowicz, A. Nogales, E. Kierzek, R. Kierzek, L. Martinez-Sobrido, D.H. Turner, Antisense Oligonucleotides Targeting Influenza A Segment 8 Genomic RNA Inhibit Viral Replication. Nucleic Acid Therapeutics, 26, 277-285 (2016).

  • E. Lenartowicz, J. Kesy, A. Ruszkowska, M. Soszynska-Jozwiak, P. Michalak, W.N. Moss, D.H. Turner, R. Kierzek, E. Kierzek, Self-Folding of Naked Segment 8 Genomic RNA of Influenza A Virus. PloS One, 11, e0148281 (2016).

  • M. Soszynska-Jozwiak, P. Michalak, W.N. Moss, R. Kierzek, E. Kierzek, A Conserved Secondary Structural Element in the Coding Region of the Influenza A Virus Nucleoprotein (NP) mRNA Is Important for the Regulation of Viral Proliferation. PloS One, 10, e0141132 (2015).

  • J. Lisowiec, M. Magner, E. Kierzek, E. Lenartowicz, R. Kierzek, Structural determinants for alternative splicing regulation of the MAPT pre-mRNA. RNA Biology, 12, 330-342, (2015).

  • R. Kierzek, D.H. Turner, E. Kierzek, Microarrays for identifying binding sites and probing structure of RNAs. Nucleic Acids Research, 43, 1-12 (2015).

Selected publications

  • SARS-CoV-2 RNA, target for inhibition of virus replication, (NSC, EXPRESS CALL TO FUND RESEARCH ON COVID-19)

  • Function of secondary structure of influenza A virus vRNA in ribonucleoprotein complex during viral life cycle, (NSC, Sonatina)

  • Modified siRNAs, ribozymes and CRISPR/Cas system as strategies for influenza A virus inhibition. Application and comparison of RNA-targeting methods, (NSC, Preludium)

Research Projects

Media Społecznościowe

Strona zrobiona w kreatorze stron internetowych WebWave

Cooperation

Institute of Bioorganic Chemistry,
Polish Academy of Sciences
Z. Noskowskiego 12/14
61-704 Poznań
tel centrala: (+48) 61 852 85 03
fax: (+48) 61 852 05 32
e-mail: ibch@ibch.poznan.pl

Sekretariat Dyrektora, tel: 61 852 89 19

Adress