Barbara Szutkowska MSc
Aleksandra Jarmołowicz MSc
Klaudia Wieczorek MSc, BEng
Aleksandra Jarmołowicz MSc
Dr. Elżbieta Lenartowicz
Dr. Julita Piasecka
RNA structural genomics of viruses
Secondary and tertiary structure of RNA and its complexes with functional proteins, other RNA and small molecules
Modulation RNA functional activities, including pathogenic RNAs involved with human diseases
RNA structural genomics of influenza virus, influence of RNA structure on virus replication
Study of structure and interactions of selected segments of influenza virus vRNA(-) and RNA(+) and important for function structural motifs of virus RNA in vitro and in vivo.
Determination in the RNA of influenza virus the regions of modified oligonucleotides and ligands strong binding
Improving microarrays mapping method with isoenergetic microarrays
Projecting and optimization of modified oligonucleotides, siRNA, ribozymes and ligands as potential inhibitors of influenza virus life cycle.
Study of structures and interactions of complexes of influenza virus vRNA(-) and virus nucleoprotein (NP).
Inhibition and regulation of influenza virus proliferation – cells studies.
Modified oligonucleotides – antisense and microarrays strategies.
Oligonucleotide carriers and bioconjugates.
CRISPR/Cas systems as strategies for replication inhibitors of RNA viruses.
Thermodynamic and comparison analysis of studied RNA, prediction of RNA secondary structure.
Studies of functional RNA structural motifs of SARS-CoV-2 virus
Modified oligonucleotides with inhibitory activities against SARS-CoV-2 virus proliferation
Searching for small molecules that inhibit SARS-CoV-2 virus proliferation
Cells tests of potential inhibitors of SARS-CoV-2 virus replication
Binding site and structural foundations for disruption of SARS-CoV-2 virus replication by inhibitors
J. Piasecka, E. Lenartowicz, M. Soszynska-Jozwiak, B. Szutkowska, R. Kierzek, E. Kierzek, RNA Secondary Structure Motifs of the Influenza A Virus as Targets for siRNA-Mediated RNA Interference. Molecular Therapy - Nucleic Acids, 19, 627–642 (2020).
J. Kesy, K.M. Patil, S.R. Kumar, Z.Y. Shu, H.Y. Yong, L. Zimmermann, A.A.L. Ong, D.F.K. Toh, M.S. Krishna, L.X. Yang, J.L. Decout, D.H. Luo, M. Prabakaran, G. Chen and E. Kierzek, A Short Chemically Modified dsRNA Binding PNA (dbPNA) Inhibits Influenza Viral Replication by Targeting Viral RNA Panhandle Structure. Bioconjugate Chemistry, 3, 931-943 (2019)
P. Michalak, M. Soszynska-Jozwiak, E. Biala, W.N. Moss, J. Kesy, B. Szutkowska, E. Lenartowicz, R. Kierzek, E. Kierzek, Secondary structure of the segment 5 genomic RNA of influenza A virus and its application for designing antisense oligonucleotides. Scientific Reports, 9, 3801 (2019)
M. Szabat, E. Kierzek, R. Kierzek, Modified RNA triplexes: Thermodynamics, structure and biological potential. Scientific Reports, 8, 13023 (2018)
M. Soszyńska-Jóźwiak, P. Michalak, W. N. Moss, R. Kierzek, J. Kesy, Elzbieta Kierzek, Influenza virus segment 5 (+)RNA - secondary structure and new targets for antiviral strategies. Scientific Reports, 7: 15041 (2017)
A Dembska, E. Kierzek, B. Juskowiak, Studying the influence of stem composition in pH-sensitive molecular beacons onto their sensing properties. Analytica Chimica Acta, 990, 157-167 (2017)
Ruszkowska, E. Lenartowicz, W. N. Moss, R. Kierzek and E. Kierzek, Secondary structure model of the naked segment 7 influenza A virus genomic RNA. Biochemical Journal, 473, 4327-4348 (2016).
E. Lenartowicz, A. Nogales, E. Kierzek, R. Kierzek, L. Martinez-Sobrido, D.H. Turner, Antisense Oligonucleotides Targeting Influenza A Segment 8 Genomic RNA Inhibit Viral Replication. Nucleic Acid Therapeutics, 26, 277-285 (2016).
E. Lenartowicz, J. Kesy, A. Ruszkowska, M. Soszynska-Jozwiak, P. Michalak, W.N. Moss, D.H. Turner, R. Kierzek, E. Kierzek, Self-Folding of Naked Segment 8 Genomic RNA of Influenza A Virus. PloS One, 11, e0148281 (2016).
M. Soszynska-Jozwiak, P. Michalak, W.N. Moss, R. Kierzek, E. Kierzek, A Conserved Secondary Structural Element in the Coding Region of the Influenza A Virus Nucleoprotein (NP) mRNA Is Important for the Regulation of Viral Proliferation. PloS One, 10, e0141132 (2015).
J. Lisowiec, M. Magner, E. Kierzek, E. Lenartowicz, R. Kierzek, Structural determinants for alternative splicing regulation of the MAPT pre-mRNA. RNA Biology, 12, 330-342, (2015).
R. Kierzek, D.H. Turner, E. Kierzek, Microarrays for identifying binding sites and probing structure of RNAs. Nucleic Acids Research, 43, 1-12 (2015).
SARS-CoV-2 RNA, target for inhibition of virus replication, (NSC, EXPRESS CALL TO FUND RESEARCH ON COVID-19)
Function of secondary structure of influenza A virus vRNA in ribonucleoprotein complex during viral life cycle, (NSC, Sonatina)
Modified siRNAs, ribozymes and CRISPR/Cas system as strategies for influenza A virus inhibition. Application and comparison of RNA-targeting methods, (NSC, Preludium)
Institute of Bioorganic Chemistry,
Polish Academy of Sciences
Z. Noskowskiego 12/14
tel centrala: (+48) 61 852 85 03
fax: (+48) 61 852 05 32
Sekretariat Dyrektora, tel: 61 852 89 19