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Europejskie Centrum Bioinformatyki i Genomiki

Instytut Informatyki Politechniki Poznańskiej

Wielkopolskie Centrum Zaawansowanych Technologii

Institute of Bioorganic Chemistry,
Polish Academy of Sciences
Z. Noskowskiego 12/14
61-704 Poznań
tel centrala: (+48) 61 852 85 03
fax: (+48) 61 852 05 32
e-mail: ibch@ibch.poznan.pl

Sekretariat Dyrektora, tel: 61 852 89 19

Department of RNA Metabolism

Dr. habil. Zbigniew Warkocki

Head of Department
zwarkocki@ibch.poznan.pl
ext. 1263

Dmytro Pandakov MSc

PhD student

Martyna Kordyś MSc

specialist biologist

Dr. Piotr Zawierucha

adiunkt

• Warkocki Z, Liudkovska V, Gewartowska O, Mroczek S, Dziembowski A.Terminal nucleotidyl transferases (TENTs) in mammalian RNA metabolism.Philos Trans R Soc Lond B Biol Sci. 2018 Nov 5;373(1762). https://doi.org/10.1098/rstb.2018.0162 PMID:30397099

• Warkocki, Z., Krawczyk, PS., Adamska, D., Bijata, K., Garcia-Perez, JL., Dziembowski, A. Uridylation by TUT4/7 restricts retrotransposition of human LINE-1s.Cell 2018 Sep 6;174(6):1537-1548.e29. https://doi.org/10.1016/j.cell.2018.07.022 (2018)

• Razew, M., Warkocki, Z., Taube, M., Kolondra, A., Czarnocki-Cieciura, M., Nowak, E., Labedzka-Dmoch, K., Kawinska, A., Piatkowski, J., Golik, P., et al.Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components.Nature Communications 9, 97. PMID: 29311576 (2018)

• Szczesny, R.J., Kowalska, K., Klosowska-Kosicka, K., Chlebowski, A., Owczarek, E.P., Warkocki, Z., Kulinski, T.M., Adamska, D., Affek, K., Jedroszkowiak, A., et al.Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.PLoS ONE 13, e0194887 PMID: 29590189 (2018).

• Łabno, A., Warkocki, Z., Kuliński, T., Krawczyk, P.S., Bijata, K., Tomecki, R., and Dziembowski, A.Perlman syndrome nuclease DIS3L2 controls cytoplasmic non-coding RNAs and provides surveillance pathway for maturing snRNAs.Nucleic Acids Research 44, 10437–10453. PMID: 27431325 (2016).

• Warkocki, Z., Schneider, C., Mozaffari-Jovin, S., Schmitzová, J., Höbartner, C., Fabrizio, P., and Lührmann, R.The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome.Genes & Development 29, 94–107. PMID: 25561498 (2015)

• Mechanisms of human gene expression regulation by uridylation. NCN SONATA - 13 

• Investigation of LINE-1 biology. NCN OPUS-17

• RNA metabolism,

• Posttranscriptional regulation of gene expression,

• RNA uridylation,

• Polyadenylation,

• Human retrotranspozons LINE-1 and Alu,

• TAIL-seq, 3’ RACE-seq

Research in the Department of RNA Metabolism concentrates on molecular mechanisms of RNA metabolism in humans. 

Leading topics are (A) investigation of enzymes catalysing RNA 3’ end uridylation and effects of uridylation on different RNA species in human cells and (B) posttranscriptional regulation of retrotransposons.     

RNA uridylation depends on non-templated addition of uridines to RNA 3’ end. Uridylation is a common modification in different RNA species including mRNA, snRNA, tRNA, rRNA, noncoding RNA and different short RNAs. While in most cases uridylation leads to destabilisation of RNA or participates in its decay, the repertoire in which uridylation can impact RNA function is broader including a well-established role of monouridylation in miRNA biogenesis and a recently discovered role of uridylation in restriction of retrotransposition by interference in reverse transcription of the retrotransposon.

Repetitive sequences constitute nearly half of the human genome. Among these LINE-1 and Alu retrotransposons can still mobilize in modern humans leading to potentially mutagenic insertions. Moreover increased LINE-1 and Alu expression might result in neurodegeneration and cancer. While epigenetic silencing pathways have been studied thoroughly, post-transcriptional mechanisms and their consequences to human health are not well understood presenting a scientific challenge.

We use human model cell lines and molecular biological, biochemical, transcriptomic and proteomic approaches to reveal molecular mechanism of posttranscriptional gene regulation by uridylation and the molecular mechanisms of retrotransposons.
 

dr Damian Janecki

adiunkt

Sen Raneet
PhD student