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Europejskie Centrum Bioinformatyki i Genomiki

Instytut Informatyki Politechniki Poznańskiej

Wielkopolskie Centrum Zaawansowanych Technologii

Institute of Bioorganic Chemistry,
Polish Academy of Sciences
Z. Noskowskiego 12/14
61-704 Poznań
tel centrala: (+48) 61 852 85 03
fax: (+48) 61 852 05 32
e-mail: ibch@ibch.poznan.pl

Sekretariat Dyrektora, tel: 61 852 89 19

Department of Non-coding RNAs

Dr. Monika Piwecka

Head of Department
monika.piwecka@ibch.poznan.pl
Phone:  61 8291855

Ayça Olcay, MS

PhD student

Marta Pietras, MS Eng.

PhD student

Dr. Monika Przybył

senior biologist

Adrian Sobusiak
biologist

• Kocks C, Boltengagen A, Piwecka M, Rybak-Wolf A, Rajewsky N. Single-Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as. Methods Mol Biol. 2018;1724:77-96.

• Piwecka M*, Glažar P*, Hernandez-Miranda LR*, Memczak S, Wolf SA, Rybak-Wolf A, Filipchyk A, Klironomos F, Cerda Jara CA, Fenske P, Trimbuch T, Zywitza V, Plass M, Schreyer L, Ayoub S, Kocks C, Kühn R, Rosenmund C, Birchmeier C, Rajewsky N. Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function. Science 2017 Sep 22;357(6357).

• Belter A*, Rolle K*, Piwecka M*, Fedoruk-Wyszomirska A, Naskręt-Barciszewska MZ, Barciszewski J. Inhibition of miR-21 in glioma cells using catalytic nucleic acids. Sci Rep. 2016 Apr 15, 6:24516.

• Rolle K*, Piwecka M*, Belter A*, Wawrzyniak D, Jeleniewicz J, Barciszewska MZ, Barciszewski J. The Sequence and Structure Determine the Function of Mature Human miRNAs. PLoS One. 2016 Mar 31; 11(3):e0151246.

• Piwecka M*, Rolle K*, Belter A, Barciszewska AM, Żywicki M, Michalak M, Nowak S, Naskręt-Barciszewska MZ, Barciszewski J. Comprehensive analysis of microRNA expression profile in malignant glioma tissues. Mol Oncol. 2015 Aug; 9(7):1324-40.

• Sowińska-Seidler A, Piwecka M, Olech E, Socha M, Latos-Bieleńska A, Jamsheer A. Hyperosmia, ectrodactyly, mild intellectual disability, and other defects in a male patient with an X-linked partial microduplication and overexpression of the KAL1 gene. J Appl Genet. 2015 May; 56(2):177-84.

We are currently specifically focusing on circular RNAs (circRNAs), miRNAs, and some lncRNAs. In our projects, we apply cutting-edge new technologies, such as single-cell RNA sequencing (scRNA-seq) and RNA imaging at a single-molecule resolution, knockdown studies of selected ncRNAs with CRISPR-Cas13 technology along with developing new methods for studying RNA: protein interactions. We take advantage of interdisciplinary efforts of molecular, cellular, and systems biology as well as neuroscience to address the question of how ncRNAs shape the regulatory networks in neurons and other neural cell types.
 

Funding

• NCN, Opus (2021-2024) – project „Non-coding RNAs at single-cell resolution in the pituitary gland and their role in the regulation of gene expression”

• NCN, Sonata Bis 8 (2019-2023) – project „Functional implications of brain-enriched circular RNAs”

• NAWA, Polish Returns 2019 (2019-2023) – project „Deciphering networks of regulatory RNAs in the central nervous system”

• regulation of gene expression,
• RNA biology,
• non-coding RNAs,
• circular RNAs (circRNAs), miRNAs, lncRNAs,
• RNA:protein interactions,
• transcriptomics,
• scRNA-seq,
• neuroscience,
• systems biology

We are a group of researchers interested in uncovering functions of non-coding RNAs in mammalian neurons, glial cells and neuroendocrine system. 

Our studies concern:
•    expression patterns of ncRNAs in single cells of the central nervous system, among its cell types, and subtypes, 
•    subcellular localization of regulatory RNAs in neurons and neuroglia, 
•    ncRNA: protein interactions,
•    regulatory ncRNA networks, 
•    functional aspects of ncRNA,
•    deregulation of non-coding transcriptome in CNS/neuroendocrine system pathologies.

Marcin Koliński
Postdoc