Laboratory of Subcellular Structures Analyses

Prof. Eliza Wyszko

Head of Laboratory

eliza.wyszko@ibch.poznan.pl
ext. 1141

Research Projects

Keywords

low molecular compounds,
nucleosides,
DNA modifications,
catalytic nucleic acids,
gene expression,
confocal microscopy,
flow cytometry,
real time PCR,
xCELLigence,
CO2 incubator,
laminar flow cabinet,
high-performance liquid chromatography,
HPLC,
MS

Offer

Confocal microscopy:

Visualization of intravital and fixed preparations,
Analysis of fluorochromes, ex. 470-670 nm and 405 nm,
2D and 3D analysis of images,
FRET, FRAP,
Analysis of fluorescence intensity,
Colocalization,

Flow cytometry:

Analysis of cell cycle,
Analysis of cell toxicity (apoptosis, necrosis),
Immunofluorescent staining,
Analysis of oxidative stress,
Cell sorting,

System xCELLigence:

Real time analysis of cell proliferation or migration,
Termocycler LightCycler 480,
Real time PCR with fluorescent probes,
HRM analysis of point mutations,
Analysis of methylation level and genotyping,

High-performance liquid chromatography (HPLC):

Analysis of nucleic acid modifications in eukaryotic cells.
Possibility of carrying out the entire experimental process including cell culturing and the use of specialized equipment by PASS.

Equipment

Confocal microscope Leica TCS SP5 II with white laser and intravital chamber with LAS AF SP5 software and LAS X SP8 software with deconvolution
DMI 4000B Leica microscope: fluorescence, bright field
Stereoscopic microscope Leica
High-performance liquid chromatography (HPLC) with detectors

■

UV,
■

fluorescent (FLD),
■

electrochemical (ED) and mass (MSI)

FACSCalibur flow cytometer (Becton Dickinson)
Accuri C6 flow cytometer (Becton Dickinson)
Cell harvester (96 wells) Perkin Elmer
Real time PCR LightCycler 480 II Roche
xCELLignce system
Cell culture CO2 incubator INCO 153 Memmert
Laminar flow cabinet BioBan 48 for sterile cell culturing

Research activity

Kinetin riboside and its derivatives - analysis of apoptotic properties and mechanism of action in brain tumor cells. NSC – OPUS 7
Anti-aging properties of 4-N-furfyrylocytosine in age-differentiated eukaryotic cells, budding yeast and mouse model of aging. NSC – OPUS 13
The regulation of neuroactive beta-carboline levels in diet based on the coffee substitute. NSC – OPUS 10 (cooperation with Poznań University of Life Sciences)

Selected publications

Skupińska M, Stępniak P, Łętowska I, Rychlewski L, Barciszewska M, Barciszewski J,Giel-Pietraszuk M.Natural Compounds as Inhibitors of Tyrosyl-tRNA SynthetaseMicrob Drug Resist. 23:308-320 (2017)
Orlicka-Płocka M, Gurda D, Fedoruk-Wyszomirska A, Smolarek I, Wyszko E.Circulating microRNAs in Cardiovascular DiseasesActa Biochim Pol. 63:725-729 (2016)
Belter A, Rolle K, Piwecka M, Fedoruk-Wyszomirska A, Naskręt-Barciszewska MZ, Barciszewski J.Inhibition of miR-21 in glioma cells using catalytic nucleic acidsSci Rep. 15:24516 (2016)
Gabryelska MM, Wyszko E, Barciszewski J.RNA dactyloscopyActa Biochim Pol. 3:785-787 (2016)
Fedoruk-Wyszomirska A, Markiewicz WT, Barciszewski J.New inhibitors of DNA methyltransferase 1 based on cytosine scaffoldBioTechnologia 97:323-325 C (2016)
Fedoruk-Wyszomirska A, Szymański M, Głodowicz P, Gabryelska M, Wyszko E,Estrin WJ, Barciszewski J.Inhibition of HIV-1 gp41 expression with hammerhead ribozymesBiochem J. 471:53-66 (2015)
Kucinska M, Skupin-Mrugalska P, Szczolko W, Sobotta L, Sciepura M, Tykarska E, Wierzchowski M, Teubert A, Fedoruk-Wyszomirska A, Wyszko E, Gdaniec M, Kaczmarek M, Goslinski T, Mielcarek J, Murias M.Phthalocyanine derivatives possessing 2-(morpholin-4-yl)ethoxy groups as potential agents for photodynamic therapyJ Med Chem. 58:2240-2255 (2015)
Mileshina D, Niazi AK, Wyszko E, Szymanski M, Val R, Valentin C, Barciszewski J, Dietrich A.Mitochondrial targeting of catalytic RNAs. MethodsMol Biol. 1265:227-254 (2015)
Barciszewska AM, Gurda D, Głodowicz P, Nowak S, Naskręt-Barciszewska MZ.A New Epigenetic Mechanism of Temozolomide Action in Glioma CellsPLoS One. 2015, 10: e0136669
Gurda D, Handschuh L, Kotkowiak W, Jakubowski H.Homocysteine thiolactone and N-homocysteinylated protein induce pro-atherogenic changes in gene expression in human vascular endothelial cellsAmino Acids. 47:1319-1339 (2015)

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Laboratory of Subcellular Structures Analyses

Researchers:

Technical Staff:

PhD students:

Research Projects

Keywords

Offer

Equipment

Research activity

Selected publications

Adres

Address

Laboratory of Subcellular Structures Analyses

Researchers:

Technical Staff:

PhD students:

Research Projects

Keywords

Rys. 1

Rys. 2.

Rys. 3.

Rys.4.

Rys. 5.

Offer

Equipment

Research activity

Selected publications

Adres

Address