Laboratory of Subcellular Structures Analyses

prof. dr hab. Eliza Wyszko

Prof. Eliza Wyszko

Head of Laboratory
ext. 1141

Research Projects

  • low molecular compounds,
  • nucleosides,
  • DNA modifications,
  • catalytic nucleic acids,
  • gene expression,
  • confocal microscopy,
  • flow cytometry,
  • real time PCR,
  • xCELLigence,
  • CO2 incubator,
  • laminar flow cabinet,
  • high-performance liquid chromatography,
  • HPLC,
  • MS


Confocal microscopy:

  • Visualization of intravital and fixed preparations,
  • Analysis of fluorochromes, ex. 470-670 nm and 405 nm,
  • 2D and 3D analysis of images,
  • Analysis of fluorescence intensity,
  • Colocalization,

Flow cytometry:

  • Analysis of cell cycle,
  • Analysis of cell toxicity (apoptosis, necrosis),
  • Immunofluorescent staining,
  • Analysis of oxidative stress,
  • Cell sorting,

System xCELLigence:

  • Real time analysis of cell proliferation or migration,
  • Termocycler LightCycler 480,
  • Real time PCR with fluorescent probes,
  • HRM analysis of point mutations,
  • Analysis of methylation level and genotyping,

High-performance liquid chromatography (HPLC):

  • Analysis of nucleic acid modifications in eukaryotic cells.
  • Possibility of carrying out the entire experimental process including cell culturing and the use of specialized equipment by PASS.
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