Laboratory of Invertebrate Model Organisms

Dr. hab. Agata Tyczewska, prof. IBCH PAS

Head of Laboratory

agatat@ibch.poznan.pl
ext. 1134

Researchers:

Kamilla Grzywacz MSc, BEng, prof. IBCH PAS

Dr. Daria Sobańska
adiunkt

Technical Staff:

Kamila Pepłowska MSc. BEng.
biologist

PhD students:

Alicja Rzepczak MSc
PhD student

Kinga Plawgo MSc
PhD student

Piotr Pietras MSc. BEng.
PhD student

Anna Wasilewska-Burczyk MSc
PhD student

Research Projects

Keywords

Caenorhabditis elegans,
C. elegans,
aging,
tRNA-derived fragments (tRF),
microinjection,
MediaJet,
Mediaclave,
Tissue Lyser,
GMO,
PC-100 micro needle puller,
nutrient media Integra,
biotechnology,
bioeconomy,
GMO legislation,
public perception of GMO,
intellectual property rights (IPR)

Offer

Nutrient media sterilization (from 1 l to 10 l)
Petri plates pouring, up to 900 plates per hour (Ø 35 mm, 60 mm, 90 mm)
Microneedle preparation
Microinjections (possibility to use with C. elegans, cell nuclei and cytomplasm of adherent cells, pronucleuses of fertilized mouse oocytes, Xenopus laevis oocytes, fish embryos in early development phases)

Equipment

Mediaclave 10, Integra
MediaJet vario, with sets for Ø 35 mm, 60 mm, 90 mm Petri plates, Integra
PC-100 microneedle puller, Narishige
Axio Vert.A1 microscope (Zeiss), with the Eppendorf microinjection set mounted:
the InjectMan ®4 micromanipulator with dynamic motion control and the programmable FemtoJet ®4i micro diaphragm with integrated pressure supply
Tissue Lyser with 2 x 24 Adapter Set for 2 ml tubes, and Grinding Jar Set (stainless steel, 2 x 10 ml)

Research activity

Low-molecular weight drug screening using C. elegans.
Identification and characterization of tRNA-derived fragments (tRFs) during aging in Caenorhabditis elegans.
Social and legal aspects of biotechnology: intellectual property, legislation, social perception
Analysis of the condition of Polish, European and global biotechnology and bioeconomy

Selected publications

Pietras PJ, Wasilewska-Burczyk A, Pepłowska K, Marczak Ł, Tyczewska A, Grzywacz K (2024) Dynamic protein composition of Saccharomyces cerevisiae ribosomes in response to multiple stress conditions reflects alterations in translation activity. Int J Biol Macromol 268(Pt 2): 132004 DOI: 10.1016/j.ijbiomac.2024.132004
Tyczewska A, Rzepczak A, Sobańska D, Grzywacz (2023) The emerging roles of tRNAs and tRNA-derived fragments during aging: Lessons from studies on model organisms. Ageing Research Reviews 85: 101863 https://doi.org/10.1016/j.arr.2023.101863
Aygün I, Rzepczak A, Miki TS (2023) A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans. Genet Mol Biol 46(2): e20220328 https://doi.org/10.1590/1678-4685-GMB-2022-0328
Tyczewska A, Twardowski T, Woźniak-Gientka E (2023) Agricultural biotechnology for sustainable food security. Trends in Biotechnology 41(3): 331-334 https://doi.org/10.1016/j.tibtech.2022.12.013
Tyczewska A, Grzywacz K (2022) tRNA-derived fragments as new players in regulatory processes in yeast. Yeast 40(8): 283-289 https://doi.org/10.1002/yea.3829
Machtel P, Wasilewska-Burczyk A, Zacharjasz J, Grzywacz K (2022) PTT-quant: a new method for direct identification and absolute quantification of premature transcription termination events, following the example of bacterial riboswitches. Appl Microbiol Biotechnol 106(4): 1557-1570 DOI: 10.1007/s00253-022-11809-1
Sobanska D, Komur AA, Chabowska-Kita A, Gumna J, Kumari P, Pachulska-Wieczorek K, Ciosk R (2022) The silencing of ets-4 mRNA relies on the functional cooperation between REGE-1/Regnase-1 and RLE-1/Roquin-1. Nucleic Acids Research 50: 8226-8239 https://doi.org/10.1093/nar/gkac609
Pekec T, Lewandowski J, Komur AA, Sobanska D, Guo Y, Switonska-Kurkowska K, Malecki JM, Dubey AA, Pokrzywa W, Frankowski M, Figiel M, Ciosk R (2022) Ferritin-mediated iron detoxification promotes hypothermia survival in Caenorhabditis elegans and murine neurons. Nature Communications 13: 4883 https://doi.org/10.1038/s41467-022-32500-z
Woźniak-Gientka E, Tyczewska A, Perisic M, Beniermann A, Eriksson D, Vangheluwe N, Gheysen G, Cetiner S, Abiri N, Twardowski T (2022) Public perception of plant gene technologies worldwide in the light of food security. GM Crops & Food 13(1): 218-241 https://doi.org/10.1080/21645698.2022.2111946
Woźniak E, Tyczewska A, Twardowski T (2021) Bioeconomy development factors in the European Union and Poland. New Biotechnology 60: 2-8 DOI: 10.1016/j.nbt.2020.07.004
Woźniak E, Tyczewska A, Twardowski T (2021) A shift towards biotechnology: Social opinion in the EU. Trends Biotechnol 39(3): 214–218 DOI: 10.1016/j.tibtech.2020.08.001
Tyczewska A, Woźniak E, Gracz J, Kuczyński J, Twardowski T (2018) Towards Food Security: Current State and Future Prospects of Agrobiotechnology. Trends Biotechnol 36(12): 1219-1229 DOI: 10.1016/j.tibtech.2018.07.008

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Laboratory of Invertebrate Model Organisms

Researchers:

Technical Staff:

PhD students:

Research Projects

Keywords

Offer

Equipment

Research activity

Selected publications

Adres

Address

Laboratory of Invertebrate Model Organisms

Researchers:

Technical Staff:

PhD students:

Research Projects

Keywords

Sample Title

Sample Title

Offer

Equipment

Research activity

Selected publications

Adres

Address