Laboratory of Plant Breeding

Dr. Aleksandra Pawela

Dr. ​Aleksandra Pawela

Head of Laboratory


apawela@ibch.poznan.pl
ext. 1169

Research and technical employees

Dr. Mariola Piślewska-Bednarek
główny specjalista ds. środowiskowej aparatury badawczej

Technical Stuff

Hanna Glapiak
biolog

Dr. Paweł Stróżycki
starszy specjalista biolog

mgr inż. Sylwia Biernacka Nowak
specjalista biolog

Research Projects

Keywords
  • Model organisms
  • Brassicaceae
  • Fabaceae
  • Plant cel lines
  • Plant tissue cultures
  • Isolation and transformation of protoplast
  • Plant transformation using vector methods
  • Plant transformation using vectorless methods (mikro-injection) 
  • Transgenic plants
  • Optimization of growing conditions
  • Microscopic observations
Plant species:
  • Arabidopsis thaliana (Brassicaceae)
  • Capsella rubella (Brassicaceae)
  • Capsella grandiflora (Brassicaceae)
  • Neslia paniculata (Brassicaceae)
  • Camelina sativa (Brassicaceae)
  • Brassica rapa (Brassicaceae)
  • Medicago truncatula (Fabaceae)
  • Glycine max (Fabaceae)
  • Lupinus albus (Fabaceae)
  • Hordeum vulgare (Poaceae)
  • Nicotiana benthamiana (Solanaceae)
  • Nicotiana tabacum cultivar Bright Yellow – 2 (cell line)

Offer

The Laboratory offers a cultivation of in vitro plant cell and tissue cultures as well as plant cultivation in phytotron chambers. Specialized infrastructure allows for precise control of temperature, humidity and lighting of the particular cultures. The Laboratory uses a number of plant cell transformation methods while ensuring safe work with transgenic material. Additional equipment, including incubators, a fluorescence microscope, an analytical balance with a deionization system, an automated system for sterilization and pouring of culture media allows for a wide range of experiments. All rooms and equipment of the Laboratory are subject to strict sterility rules (UV sterilization systems, ozonation). An advanced, regularly serviced filtration and water treatment system allows for work with three types of water with different purity levels. Laboratory employees, based on their knowledge and experience, provide support in optimizing the conditions for cultivating given plant species and minimizing infections in phytotron cultures and in vitro cultures. The main methods of plant transformation used in the Laboratory are vector methods using Agrobacterium tumefaciens (the Laboratory offers access to various Agrobacterium strains). In addition, the Laboratory has a wide database of binary vectors in various cloning systems and will provide all support in the preparation of gene constructs, including the choice of a selection gene.

Selected publications

  • Konrad Pakuła; Carlos Sequeiros-Borja; Wanda Biała-Leonhard; Aleksandra Pawela; Joanna Banasiak; Aurélien Bailly; Marcin Radom; Markus Geisler; Jan Brezovsky; Michał Jasiński. Restriction of access to the central cavity is a major contributor to substrate selectivity in plant ABCG transporters. Cellular and Molecular Life Sciences (2023).
  • Czerniawski, P., Piślewska-Bednarek, M., Piasecka, A., Kułak, K., Bednarek, P. Loss of MYB34 Transcription Factor Supports the Backward Evolution of Indole Glucosinolate Biosynthesis in a Subclade of the Camelineae Tribe and Releases the Feedback Loop in This Pathway in Arabidopsis. Plant and Cell Physiology, 2023, 64(1), pp. 80–93
  • Piasecka, A., Sawikowska, A., Jedrzejczak-Rey, N., Piślewska-Bednarek, M., Bednarek, P. Targeted and Untargeted Metabolomic Analyses Reveal Organ Specificity of Specialized Metabolites in the Model Grass Brachypodium distachyon. Molecules, 2022, 27(18), 5956
  • Kosaka A, Pastorczyk M, Piślewska-Bednarek M, Nishiuchi T, Ono E, Suemoto H, Ishikawa A, Frerigmann H, Kaido M, Mise K, Bednarek P, Takano Y. Tryptophan-derived metabolites and BAK1 separately contribute to Arabidopsis postinvasive immunity against Alternaria brassicicola. Sci Rep. 2021 Jan 15;11(1):1488. doi: 10.1038/s41598-020-79562-x. PMID: 33452278; PMCID: PMC7810738.
  • Jarzyniak K, Banasiak J, Jamruszka T, Pawela A, Di Donato M, Novák O, Geisler M, Jasiński M. Early stages of legume–rhizobia symbiosis are controlled by ABCG-mediated transport of active cytokinins. Nat. Plants (2021).
  • Pastorczyk M, Kosaka A, Piślewska-Bednarek M, López G, Frerigmann H, Kułak K, Glawischnig E, Molina A, Takano Y, Bednarek P. The role of CYP71A12 monooxygenase in pathogen-triggered tryptophan metabolism and Arabidopsis immunity. New Phytol. 2020 Jan;225(1):400-412. doi: 10.1111/nph.16118. Epub 2019 Sep 21. PMID: 31411742.
  • Pawela, A., Banasiak J., Biała, W., Martinoia E., Jasinski M., MtABCG20 is an ABA exporter influencing root morphology and seed germination of Medicago truncatula The Plant Journal 2019 DOI: 10.1111/tpj.14234
  • Piślewska-Bednarek M, Nakano RT, Hiruma K, Pastorczyk M, Sanchez-Vallet A, Singkaravanit-Ogawa S, Ciesiołka D, Takano Y, Molina A, Schulze-Lefert P, Bednarek P. Glutathione Transferase U13 Functions in Pathogen-Triggered Glucosinolate Metabolism. Plant Physiol. 2018 Jan;176(1):538-551. doi: 10.1104/pp.17.01455. Epub 2017 Nov 9. PMID: 29122987; PMCID: PMC5761798.
  • Biała, W., Banasiak, J., Jarzyniak, K., Pawela, A., Jasiński, M. Medicago truncatula ABCG10 is a transporter of 4-coumarate and liquiritigenin in the medicarpin biosynthetic pathway Journal of Experimental Botany 2017 68(12), pp. 3231-3241
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