Department of Ribonucleoprotein Biochemistry

Dr. hab. Anna Kurzyńska-Kokorniak, prof. IBCH PAS

Head of Department

akurzyns@ibch.poznan.pl
ext. 1264

Research activity

Research area

the role of RNA/ DNA in the activity and functions of nucleic acid binding proteins
mechanisms involved in the regulation of gene expression
ribonuclease Dicer variants (gene, transcript, protein)
endogenous factors regulating the activity of ribonuclease Dicer
designing and testing of inhibitors against Dicer-type ribonucleases
the activity of Dicer-type ribonucleases in the context of viral infections, viral miRNAs
unknown areas of activity of Dicer-type ribonucleases: ribonuclease Dicer as a nucleic acid annealer, binding of G-quadruplex structures
diseases linked to malfunction of Dicer
structural studies of the RNA•Dicer and DNA•Dicer complexes
inhibition of the SARS-CoV-2 polymerase complex

Keywords:

nucleic acid binding proteins
Dicer-type ribonucleases
SARS-CoV-2 polymerase complex
regulatory RNAs
RNA-protein interactions, DNA-protein interactions
regulation of the activity of Dicer-type ribonucleases
inhibitors of the SARS-CoV-2 polymerase complex
regulation of gene expression

Research Projects

2021/41/B/NZ2/03781 Bliskie spotkania trzeciego stopnia: co dzieje się kiedy rybonukleaza Dicer napotyka w komórce RNA i DNA przyjmujące struktury G-kwadrupleksów. NCN - OPUS 21 (AKK)
2016/22/E/NZ1/00422 Niepoznane obszary aktywności ludzkiej rybonukleazy Dicer. NCN – SONATA BIS 6 (AKK)
2021/41/N/NZ2/03849 Komórkowa sieć oddziaływań pomiędzy RNA i domeną helikazową rybonukleazy Dicer człowieka. NCN - PRELUDIUM 20 (KC)
2024/08/X/NZ1/00899 Otrzymanie dwóch wariantów rybonukleazy Dicer organizmu modelowego Xenopus laevis – przygotowanie do badań aktywności biochemicznych i analiz strukturalnych. NCN – MINIATURA 8 (ASz)
2021/05/X/NZ1/01778 Poznanie strukturalnych uwarunkowań oddziaływań pomiędzy kasetą PPC ludzkiej rybonukleazy Dicer a cząsteczkami RNA i DNA przyjmującymi struktury G-kwadrupleksu. NCN - MINIATURA 5 (KSz)
POIR.01.01.01-00-2450/20 Opracowanie wysokoprzepustowego systemu umożliwiającego szybką ocenę aktywności biologicznych związków nakierowanych na kompleks replikacyjny SARS-CoV-2, wytypowanie związków o najwyższym potencjale terapeutycznym i ocena ich cytotoksyczności wobec komórek ludzkich. NCBR - Szybka Ścieżka 5 (projekt realizowany w konsorcjum, lider projektu: ideas4biology Sp. z o. o.; kierownik zadań z ramienia ICHB PAN: AKK)

Selected publications

Koralewska N, Corradi E, Milewski MC, Masante L, Szczepanska A, Kierzek R, Figlerowicz M, Baudet ML, Kurzynska-Kokorniak A*. Short 2'-O-methyl/LNA oligomers as highly-selective inhibitors of miRNA production in vitro and in vivo . Nucleic Acids Res. 2024; 52(10):5804-5824. doi: 10.1093/nar/gkae284.
Szpotkowski K, Wójcik K, Kurzyńska-Kokorniak A*. Structural studies of protein-nucleic acid complexes: A brief overview of the selected techniques. Comput Struct Biotechnol J. 2023; 21:2858-2872. doi: 10.1016/j.csbj.2023.04.028.
Kazimierczyk M, Wojnicka M, Biała E, Żydowicz-Machtel P, Imiołczyk B, Ostrowski T, Kurzynska-Kokorniak A, Wrzesinski J. Characteristics of Transfer RNA-Derived Fragments Expressed during Human Renal Cell Development: The Role of Dicer in tRF Biogenesis. Int. J. Mol. Sci. 2022; 23(7):3644. doi: 10.3390/ijms23073644.
Koralewska N, Szczepanska A, Ciechanowska K, Wojnicka M, Pokornowska M, Milewski MC, Gudanis D, Baranowski D, Nithin C, Bujnicki JM, Gdaniec Z, Figlerowicz M, Kurzynska-Kokorniak A*. RNA and DNA G-quadruplexes bind to human dicer and inhibit its activity. Cell Mol Life Sci. 2021; 78(7):3709-3724. doi: 10.1007/s00018-021-03795-w.
Szczepanska A, Wojnicka M, Kurzynska-Kokorniak A*. The Significance of the DUF283 Domain for the Activity of Human Ribonuclease Dicer. Int J Mol Sci. 2021;22(16):8690. doi: 10.3390/ijms22168690.
Ciechanowska K, Pokornowska M, Kurzyńska-Kokorniak A*. Genetic Insight into the Domain Structure and Functions of Dicer-Type Ribonucleases. Int J Mol Sci. 2021; 22(2):616. doi: 10.3390/ijms22020616.
Pokornowska M, Milewski MC, Ciechanowska K, Szczepańska A, Wojnicka M, Radogostowicz Z, Figlerowicz M, Kurzynska-Kokorniak A*. The RNA-RNA base pairing potential of human Dicer and Ago2 proteins. Cell Mol Life Sci. 2020; 77(16):3231-3244. doi: 10.1007/s00018-019-03344-6. ·
Wojnicka M, Szczepanska A, Kurzynska-Kokorniak A*. Unknown Areas of Activity of Human Ribonuclease Dicer: A Putative Deoxyribonuclease Activity . Molecules. 2020; 25(6):1414. doi: 10.3390/molecules25061414.
Milewski MC, Kamel K, Kurzynska-Kokorniak A, Chmielewski MK, Figlerowicz M. EvOligo – a novel software to design and group libraries of oligonucleotides applicable for nucleic acid-based experiments. J Comput Biol. 2017; 24(10):1014-1028. doi: 10.1089/cmb.2016.0154.
Mickiewicz A, Sarzyńska J, Miłostan M, Kurzynska-Kokorniak A, Rybarczyk A, Łukasiak P, Kuliński T, Figlerowicz M, Błażewicz J. Modeling of the catalytic core of Arabidopsis thaliana Dicer-like 4 protein and its complex with double-stranded RNA. Comput Biol Chem. 2017; 66:44-56. doi: 10.1016/j.compbiolchem.2016.11.003.
Kurzynska-Kokorniak A*, Pokornowska M, Koralewska N, Hoffmann W, Bienkowska-Szewczyk K, Figlerowicz M*. Revealing a new activity of the human Dicer DUF283 domain in vitro. Sci Rep. 2016; 6:23989. doi: 10.1038/srep23989.
Kurzynska-Kokorniak A, Koralewska N, Pokornowska M, Urbanowicz A, Tworak A, Mickiewicz A, Figlerowicz M. The many faces of Dicer: the complexity of the mechanisms regulating Dicer gene expression and enzyme activities. Nucleic Acids Res. 2015; 43(9):4365-80. doi: 10.1093/nar/gkv328.

* corresponding author

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Department of Ribonucleoprotein Biochemistry

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Department of Ribonucleoprotein Biochemistry

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