5ʹ-phosphorylated oligonucleotides are important tools in molecular techniques finding various applications as linkers, adapters, in cloning, gene regulation, as well as in enzymatic ligation. In collaboration with FutureSynthesis sp.z o.o., M.Sc. Dominika Krygier and Ph.D. Marcin Chmielewski from the Department of Biopolymer Chemistry have developed an original method for phosphorylation of oligonucleotides using a thermosensitive “trigger.” Two identical thermolabile protecting groups are removed in different times: the release of the first occurs rapidly, and the delayed deprotection of the second one allowed the development of an efficient method for the purification of 5′-phosphorylated oligonucleotides called TPG-on. Removal of the group from the final oligonucleotide is simple and fast by using microwave radiation.

https://pubs.acs.org/doi/10.1021/acs.orglett.3c03924