Laboratory of Invertebrate Model Organisms

Dr. hab. Agata Tyczewska, prof. IBCH PAS

Head of Laboratory

agatat@ibch.poznan.pl
ext. 1134

Research Projects

Keywords

Caenorhabditis elegans
C. elegans
Saccharomyces cerevisiae
S. cerevisiae
aging of organisms
regulation of gene expression by short noncoding RNAs
short noncoding RNAs derived from tRNAs (tRFs) and snoRNAs (sdRNAs)
short noncoding RNAs interacting with ribosomes (rancRNAs)

Offer

Nutrient media sterilization (from 1 l to 10 l)
Petri plates pouring, up to 900 plates per hour (Ø 35 mm, 60 mm, 90 mm)
Microneedle preparation
Microinjections (possibility to use with C. elegans, cell nuclei and cytomplasm of adherent cells, pronucleuses of fertilized mouse oocytes, Xenopus laevis oocytes, fish embryos in early development phases)

Equipment

fully equipped microorganism culturing room with BIO BAN 48 laminar flow cabinet
Mediaclave 10 Integra microbiological substrate preparation device
MediaJet Vario Integra device for automatic filling of Petri dishes in sterile conditions with carousels for plates Ø 35 mm, 60 mm, 90 mm
large capacity incubators
device for producing microneedles with a diameter of 1µm - 10 µm made of borosilicate glass PC-100 by Narishige
Zeiss Axio Vert.A1 inverted contrast fluorescence microscope, using all standard contrast techniques: bright field, phase contrast, PlasDIC, improved Hoffman modulation contrast (iHMC) and fluorescence contrast, and LED light gentle on living samples
Eppendorf microinjection kit integrated with fluorescence microscope: InjectMan 4 micromanipulator with dynamic motion control and FemtoJet 4i programmable microinjector with integrated pressure supply
Nicon SMZ25 fluorescence stereomicroscope with FHD camera (HDMI16MDPXII) – DeltaPix for intravital observations
Leica stereomicroscopes and Nikon binoculars
MiliQ water treatment station and ddH2O HydroLabsystem dokumentacji żeli, chemiluminescencji Azure Biosystems
Classic Prestige Medical tabletop autoclaves
Tissue Lyser Eppendorf medium and high-throughput low-temperature grinding homogenizer with 24 x 2ml and 2 x 10ml adapters
Eppendorf 5804R and 5424 centrifuges
Qubit 4 fluorometer from Invitrogen
Nanodrop One spectrophotometer
ProFlex and BioRad thermocyclers
a device for preparing linear sucrose and glycerol gradients
BluPAD and GeneDire transilluminators
pH meters
TransBlot Turbo and Mini Blot Module devices for transferring proteins and nucleic acids
Victor Nivo plate reader (detection modes: Abs, Lum, FI, FP, TRF, and Alpha, gas dispenser and controller, time-resolved fluorescence)

Research activity

Screening of Low Molecular Weight Drugs Using Caenorhabditis elegans
Identification and Characterization of Short Non-Coding RNAs Derived from tRNAs (tRFs) During Aging in Caenorhabditis elegans
Short RNAs Derived from tRNAs (tRFs) as Stress-Responsive Regulatory Molecules in Soybean
Changes in Ribosomal Protein Composition and Ribosome-Interacting Short Non-Coding RNAs in Saccharomyces cerevisiae in Response to Environmental Stress
Morphological Changes in Saccharomyces cerevisiae in Response to Environmental Stress

Selected publications

Pietras PJ, Wasilewska-Burczyk A, Pepłowska K, Marczak Ł, Tyczewska A, Grzywacz K (2024) Dynamic protein composition of Saccharomyces cerevisiae ribosomes in response to multiple stress conditions reflects alterations in translation activity. Int J Biol Macromol 268(Pt 2): 132004 DOI: 10.1016/j.ijbiomac.2024.132004
Tyczewska A, Rzepczak A, Sobańska D, Grzywacz (2023) The emerging roles of tRNAs and tRNA-derived fragments during aging: Lessons from studies on model organisms. Ageing Research Reviews 85: 101863 https://doi.org/10.1016/j.arr.2023.101863
Aygün I, Rzepczak A, Miki TS (2023) A germline-targeted genetic screen for xrn-2 suppressors identifies a novel gene C34C12.2 in Caenorhabditis elegans. Genet Mol Biol 46(2): e20220328 https://doi.org/10.1590/1678-4685-GMB-2022-0328
Tyczewska A, Twardowski T, Woźniak-Gientka E (2023) Agricultural biotechnology for sustainable food security. Trends in Biotechnology 41(3): 331-334 https://doi.org/10.1016/j.tibtech.2022.12.013
Tyczewska A, Grzywacz K (2022) tRNA-derived fragments as new players in regulatory processes in yeast. Yeast 40(8): 283-289 https://doi.org/10.1002/yea.3829
Machtel P, Wasilewska-Burczyk A, Zacharjasz J, Grzywacz K (2022) PTT-quant: a new method for direct identification and absolute quantification of premature transcription termination events, following the example of bacterial riboswitches. Appl Microbiol Biotechnol 106(4): 1557-1570 DOI: 10.1007/s00253-022-11809-1
Sobanska D, Komur AA, Chabowska-Kita A, Gumna J, Kumari P, Pachulska-Wieczorek K, Ciosk R (2022) The silencing of ets-4 mRNA relies on the functional cooperation between REGE-1/Regnase-1 and RLE-1/Roquin-1. Nucleic Acids Research 50: 8226-8239 https://doi.org/10.1093/nar/gkac609
Pekec T, Lewandowski J, Komur AA, Sobanska D, Guo Y, Switonska-Kurkowska K, Malecki JM, Dubey AA, Pokrzywa W, Frankowski M, Figiel M, Ciosk R (2022) Ferritin-mediated iron detoxification promotes hypothermia survival in Caenorhabditis elegans and murine neurons. Nature Communications 13: 4883 https://doi.org/10.1038/s41467-022-32500-z
Woźniak-Gientka E, Tyczewska A, Perisic M, Beniermann A, Eriksson D, Vangheluwe N, Gheysen G, Cetiner S, Abiri N, Twardowski T (2022) Public perception of plant gene technologies worldwide in the light of food security. GM Crops & Food 13(1): 218-241 https://doi.org/10.1080/21645698.2022.2111946
Woźniak E, Tyczewska A, Twardowski T (2021) Bioeconomy development factors in the European Union and Poland. New Biotechnology 60: 2-8 DOI: 10.1016/j.nbt.2020.07.004
Woźniak E, Tyczewska A, Twardowski T (2021) A shift towards biotechnology: Social opinion in the EU. Trends Biotechnol 39(3): 214–218 DOI: 10.1016/j.tibtech.2020.08.001
Tyczewska A, Woźniak E, Gracz J, Kuczyński J, Twardowski T (2018) Towards Food Security: Current State and Future Prospects of Agrobiotechnology. Trends Biotechnol 36(12): 1219-1229 DOI: 10.1016/j.tibtech.2018.07.008

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Laboratory of Invertebrate Model Organisms

Researchers:

Technical Staff:

PhD students:

Research Projects

Keywords

Offer

Equipment

Research activity

Selected publications

Adres

Address

Laboratory of Invertebrate Model Organisms

Researchers:

Technical Staff:

PhD students:

Research Projects

Keywords

Sample Title

Sample Title

Offer

Equipment

Research activity

Selected publications

Adres

Address