The Laboratory of Model Mammalian Organisms conducts comprehensive experiments on animals as part of projects implemented at the Institute of Bioorganic Chemistry of the Polish Academy of Sciences. We offer assistance in such aspects of scientific work as:

• planning expenses in grant applications
• designing experiments in accordance with the 3R and ARRIVE principles
• preparing an application to the Local Ethics Committee, the Ministry of the Environment or the Provincial Pharmaceutical Inspectorate
  
• organization of transport of experimental animals
• obtaining appropriate consents to work with laboratory animals
• training in basic techniques of animal work
• maintaining a colony of transgenic or wild-type mice with a database
• obtaining embryonic material at appropriate stages of development
  
• intramuscular, intraperitoneal, subcutaneous and intracranial injections
  
• microsurgery
  
• implantation of osmotic pumps subcutaneously and into ICV
  
• keeping experimental records
• behavioral tests (Rotarod, static rod, open-field, home-cage activity, phenotype scoring, grip strength, gait analysis, beaker, hindlimb, hanging wire)
• digitalization, publication-ready figures and statistical analysis of data acquired
• analysis of mouse/rat/human immunophenotype using flow cytometry
  
• intragastric administration
  
• Intratracheal intubations
  
• Uninephrectomy and acute kidney injury
• Xenograft inoculations
• mouse perfusion, organ weighing, and blood and tissue collection
  
• preparation of the entire aorta en face
  
• dissection of the brain into structures (cortex, striatum, hippocampus, cerebellum, pons, medulla, pituitary gland, olfactory bulbs
• Bronchoalveolar lavage
  
• Culture of cell lines ​HeLa, HepG2, PANC1, L929, U87, U118, U251, MRC-5, OVCAR, BEAS-2B, HFF-1, A594, MDCK, SP2/ag14, Caco-2, MDA-MB-231, hMECs and primary cells (Neurons, Astrocytes, Vascular Smooth Muscle cells [VSMCs], Macrophages, Mouse Embryonic Fibroblasts [MEFs])
• Animal immunization and antibody production
  
• Immune status and polarization of macrophages by real-time PCR
  
• Blood-brain barrier, arterial, venous and placental cell model under flow 
• Acute brain slices – ex vivo GBM invasion assay