Laboratory of Molecular Assays and Imaging

dr Dorota Kwiatek

Head of Laboratory

dkwiatek@ibch.poznan.pl
tel. 1555

Research activity

Key words

Super-resolution microscopy, MINFLUX, FLIM, STED
Single molecule imaging
High Throughput Screening
Molecular Assays Development
Cell Painting
Image Analysis
Data Analysis

Offer

The Laboratory of Molecular Assays and Imaging is part of the Centre for Chemical Biology ERIC at the Institute of Bioorganic Chemistry PAS and offers access to expertise and infrastructure for the development of molecular assays as well as performing screening, high-throughput microscopy and image analysis. It is a part of EU-OPENSCREEN-ERIC consortium which collaborates with users worldwide for identification of biologically active molecules and deconvolution of their mechanisms of action.

The infrastructure of the Laboratory is part of a platform for multidimensional imaging of biological processes, established within the framework of NEBI - National Research Center for Imaging in the Biological and Biomedical Sciences. It is equipped with an ultra-high-resolution microscopy system located in IBCH PAS. Thanks to a unique technology of MINFLUX microscope (only few in the world) the imaging with resolution below 2 nm can be performed. Our MINFLUX microscope system combines super-resolution imaging technologies, i.e. STED, FLIM, Minflux and similar.

Equipment

Minflux – ultra-high-resolution microscope (Abberior)
Opera Phenix (Perkin Elmer) High Content Screening System
Echo (Labcyte) acoustic dispenser
Multidrop combi (ThermoFisher) liquid dispenser
Hydrospeed plate washer (Tecan)
CLARIOstar Plus (BMGLabtech) and Cytation (Biotek) multimode plate readers

Projects/Tasks

Selected Projects

FENG.02.04-IP.04-0010/24-00
(POL-OPENSCREEN) Project of the Polish Platform of Screening Infrastructure for Biological Chemistry, which is part of the EU-OPENSCREEN (European Infrastructure of Open Screening Platforms for Chemical Biology European Research Infrastructure Consortium). Financed by the Ministry of Science and Higher Education
POL-OPENSCREEN 2.0 - Maintenance, Development and Sharing of the Polish Screening Infrastructure Platform for Biological Chemistry, in view of the Participation in the EU-OPENSCREEN ERIC Research Infrastructure Consortium)

Selected projects which tasks are currently carried out in the Laboratory:

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IMProving User experience, Long-term sustainability and Services of EU-OPENSCREEN (akronim IMPULSE); EU - program Horizon Europe, HORIZON- INFRA-2023-DEV-01, agreement no.: 101132028; project PI on behalf of IBCH PAS: dr hab. Jacek Kolanowski, task leader: dr Dorota Kwiatek
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Bliskie spotkania trzeciego stopnia: co dzieje się kiedy rybonukleaza Dicer napotyka w komórce RNA i DNA przyjmujące struktury G-kwadrupleksów; NCN – konkurs OPUS 21, nr projektu: 2021/41/B/NZ2/03781; project PI: dr hab. Anna Kurzyńska-Kokorniak
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Integrated Services for Infectious Disease Outbreak Research (akronim: ISIDORe); EU – program Horizon Europe, HE-HORIZON-INFRA-2021-EMERGENCY-02, nr umowy: 101046133; project PI on behalf of IBCH PAS: dr hab Jacek Kolanowski, including 2 scientific collaborations:
- Dr. Sandeep Ghorpade, University of Cape Town, pt. Identification of validated hits against Mycobacterium tuberculosis caseinolytic protease complex ClpC1P1P2 from ECBL library; task leader: dr Dorota Kwiatek
- Prof. Bart Haagmans, Department of Viroscience, Rotterdam, pt. Viral Infectiology Research with Advanced Laboratory models (ISIDORe JRA VIRAL); task leader: dr Dorota Kwiatek
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Integrated assessment and advanced characterisation neuro-nanotoxicity (akronim: iCare); EU – program Horizon Europe, HORIZON-CL4-2022-DIGITAL-EMERGING-01, agreement no. 101092971; project PI on behalf of IBCH PAS: dr hab. Jacek Kolanowski
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Poszukiwanie inhibitorów ludzkiej reduktazy δ1-pyrrolino-5-karboksylanu (PYCR1) jako cząsteczek wiodących w rozwoju nowych leków antynowotworowych; NCN –OPUS 21, project no.: 2021/43/B/NZ7/0161; project PI: dr hab. Miłosz Ruszkowski
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Alternative gene ends:the crosstalk of RNA cleavage and transcription termination (akronim: Alternative Ends); EU – program Horizon Europe, ERC-2021-STG; project PI: dr hab. Kinga Kamieniarz-Gdula, Uniwersytet im. Adama Mickiewicza w Poznaniu
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Nanoscale Hippocalcin Signaling in Long-Term Depression in Norm and Primary Dystonia; Polska Akademia Nauk, program wsparcia naukowców z Ukrainy, agreement no.: PAN.BFB.S.BWZ.405.022.2023; project PI: dr Volodymyr Cherkas

Wybrane publikacje

[1] F. Canyelles I Font, K. Żukowski, M. A. Khan, D. Kwiatek, J. L. Kolanowski, Interference of metal ions on the bioluminescent signal of firefly, Renilla, and NanoLuc luciferases in high-throughput screening assays, Front. Chem. (2024) Nov 26:12:1436389. doi: 10.3389/fchem.2024.1436389

[2] A. Wychowaniec, F. Canyelles i Font, M. A. Khan, M. Gładysz, D. Kwiatek, J.L. Kolanowski Wieloanalitowe, małocząsteczkowe sondy luminescencyjne do symultanicznego wykrywania kilku celów molekularnych w modelach komórkowych, Postępy Biochemii (2024) 10.18388/pb.2021_538

[3] N. Karczewska, M. Pyc, K. Żukowski, J. Kosman, D. Kwiatek, J.L. Kolanowski Rola i wykorzystanie badań wysokoprzepustowych w poszukiwaniu związków chemicznych o działaniu terapeutycznym, Postępy Biochemii (2024) 10.18388/pb.2021_528

[4] M.Insińska-Rak, A. Golczak, M. Gierszewski, Z. Anwar, V. Cherkas, D. Kwiatek, E. Sikorska, I. Khmelinskii, G. Burdziński, R. Cibulka, L. Mrówczyńska, J.L. Kolanowski, M. Sikorski 5-Deazaalloxazine as photosensitizer of singlet oxygen and potential redox-sensitive agent, Photochemical and Photobiological Sciences 22 (2023) 1655-1671

[5] A. Golczak, D. Prukała, E. Sikorska, M. Gierszewski, V. Cherkas, D. Kwiatek, A. Kubiak, N. Varma, T. Pędziński, S. Murphree, R. Cibulka, L. Mrówczyńska, J.L. Kolanowski, M. Sikorski, Tetramethylalloxazines as efficient singlet oxygen photosensitizers and potential redox-sensitive agents, Scientific Reports 13426 (2023)

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