Laboratory of Mass Spectrometry

Dr. Łukasz Marczak

Head of Laboratory

lukasmar@ibch.poznan.pl
Phone: 61 6653051

Research Projects

Keywords

Mass spectrometry,
chromatography,
HPLC,
GCMS,
electrophoresis,
proteomics,
metabolomics,
lipidomics,
analytical chemistry,
quantitative analysis,
atherosclerosis,
cancer,
blood,
blood plasma,
hyperhomocysteinemia,
nhomocysteinylation,
exosomes,
extracellular vesicles,
biomarker

Offer

Protein identification by MS methods
De novo analysis of peptides and protein sequences (MALDI, ISD-MALDI)
Analysis of post-translational modification of proteins by MS methods
Quantitative analysis of proteins by electrophoresis and MS methods
Determination of monoisotopic masses of low molecular weight compounds using
HR-MS methods
Structural analysis of low molecular weight compounds by tandem mass spectrometry methods
Quantitative analysis of low molecular weight compounds using MS methods
Volatile compounds analysis using GC-MSn methods

Equipment

Institute of Bioorganic Chemistry PAS:

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HPLC-MS system (Q-TOF) - Bruker micrOTOF-q
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GC-MS (TOF) - Waters GCT Premier

European Center for Bioinformatics and Genomics:

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nanoLC-MS (offline MALDI-TOF/TOF) - Proxeon nanoLC + Bruker UltrafleXtreme
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nanoLC-MS system (ion trap) - Waters nano Acquity + Bruker Amazon SL
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Nano/micro LC-MS (OrbiTrap) - Dionex RSLC nano 3000 + Thermo QExactive
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GCxGC-MS (TOF) - Leco Pegasus 4D
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GC-MS (TripleQuad) - Thermo TSQ8000
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external ion source with fraction collector - Advion NanoMate TriVersa
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2D electrophoresis system - GE Healthcare IPGphor + EttanDalt six

Research activity

Why cells become smaller to survive? Analysis of tobacco BY2 cells adapted to osmotic and salt stress in the search of key regulatory factors between energy management, molecular homeostasis and the size of the chronically stressed plant cells (consortium with Institute of Dendrology Polish Academy of Sciences, IBCH PI: Dr. Łukasz Marczak)
Identification of biomolecules released by colorectal cancer cells and detected in serum or/and exosomes (PI: Dr. Anna Wojakowska)

Selected publications

M. Pietrowska, A. Zebrowska, M. Gawin, L. Marczak, P. Sharma, S. Mondal, J. Mika, J. Polanska, S. Ferrone, J. M. Kirkwood, P. Widlak, T. L. Whiteside, Proteomic profile of melanoma cell-derived small extracellular vesicles in patients’ plasma: a potential correlate of melanoma progression. J. Extracell. VESICLES. 10 (2021), doi:10.1002/jev2.12063.
L. Marczak, J. Idkowiak, J. Tracz, M. Stobiecki, B. Perek, K. Kostka-Jeziorny, A. Tykarski, M. Wanic-Kossowska, M. Borowski, M. Osuch, D. Formanowicz, M. Luczak, Mass spectrometry-based lipidomics reveals differential changes in the accumulated lipid classes in chronic kidney disease. Metabolites. 11 (2021), doi:10.3390/metabo11050275.
A. Liskiewicz, M. Przybyla, A. Wojakowska, L. Marczak, K. Bogus, M. Nowacka-Chmielewska, D. Liskiewicz, A. Malecki, J. Barski, J. Lewin-Kowalik, M. Toborek, Physical activity reduces anxiety and regulates brain fatty acid synthesis. Mol. Brain. 13 (2020), doi:10.1186/s13041-020-00592-7.
A. Szuba, L. Marczak, I. Ratajczak, Metabolome adjustments in ectomycorrhizal Populus x canescens associated with strong promotion of plant growth by Paxillus involutus despite a very low root colonization rate. TREE Physiol. 40, 1726–1743 (2020).
A. Wojakowska, A. Zebrowska, A. Skowronek, T. Rutkowski, K. Polanski, P. Widlak, L. Marczak, M. Pietrowska, Metabolic profiles of whole serum and serum-derived exosomes are different in head and neck cancer patients treated by radiotherapy. J. Pers. Med. 10, 1–14 (2020).
M. Sikora, Ł. Marczak, J. Perła-Kajan, H. Jakubowski, Sex affects N-homocysteinylation at lysine residue 212 of albumin in mice. Sci. Rep. 9 (2019), doi:10.1038/s41598-019-38784-4.
A. Zebrowska, A. Skowronek, A. Wojakowska, P. Widlak, M. Pietrowska, Metabolome of exosomes: Focus on vesicles released by cancer cells and present in human body fluids. Int. J. Mol. Sci. 20 (2019), doi:10.3390/ijms20143461.
K. Kimakova, A. Kimakova, J. Idkowiak, M. Stobiecki, P. Rodziewicz, L. Marczak, E. Cellarova, Phenotyping the genus Hypericum by secondary metabolite profiling: emodin vs. skyrin, two possible key intermediates in hypericin biosynthesis. Anal. Bioanal. Chem. 410, 7689–7699 (2018).
M. Pietrowska, S. Funk, M. Gawin, Ł. Marczak, A. Abramowicz, P. Widłak, T. Whiteside, Isolation of exosomes for the purpose of protein cargo analysis with the use of mass spectrometry (2017), vol. 1654.
P. Kachlicki, A. Piasecka, M. Stobiecki, Ł. Marczak, Structural characterization of flavonoid glycoconjugates and their derivatives with mass spectrometric techniques. Molecules. 21 (2016), doi:10.3390/molecules21111494.
S. J. Gadher, Ł. Marczak, M. Łuczak, M. Stobiecki, P. Widlak, H. Kovarova, Proteomic landscape in central and Eastern Europe: The 9th Central and Eastern European Proteomic conference, Poznan, Poland. Expert Rev. Proteomics. 13 (2016), doi:10.1586/14789450.2016.1116391.
Ł. Marczak, P. Znajdek-Awizeń, W. Bylka, The use of mass spectrometric techniques to differentiate isobaric and isomeric flavonoid conjugates from Axyris amaranthoides. Molecules. 21 (2016), doi:10.3390/molecules21091229.
A. Wojakowska, M. Chekan, Ł. Marczak, K. Polanski, D. Lange, M. Pietrowska, P. Widlak, Detection of metabolites discriminating subtypes of thyroid cancer: Molecular profiling of FFPE samples using the GC/MS approach. Mol. Cell. Endocrinol. 417, 149–157 (2015).
M. Sikora, Ł. Marczak, J. Kubalska, Ałł. Graban, H. Jakubowski, Identification of N-homocysteinylation sites in plasma proteins. Amino Acids. 46, 235–244 (2014).
Ł. Marczak, M. Sikora, M. Stobiecki, H. Jakubowski, Analysis of site-specific N-homocysteinylation of human serum albumin in vitro and in vivo using MALDI-ToF and LC-MS/MS mass spectrometry. J. Proteomics. 74 (2011), doi:10.1016/j.jprot.2011.01.021.

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Laboratory of Mass Spectrometry

Researchers:

Technical Staff:

PhD students:

Research Projects

Keywords

Offer

Equipment

Research activity

Selected publications

Adres

Address

Laboratory of Mass Spectrometry

Researchers:

Technical Staff:

PhD students:

Research Projects

Keywords

Rys. 1

Rys. 2.

Rys. 3

Rys. 4

Offer

Equipment

Research activity

Selected publications

Adres

Address