The Laboratory supports both independent experimentation and on-demand research services. We offer state-of-the-art infrastructure and technical expertise for working with animal cells, including human and insect cell lines. Our facility includes specialized cell culture rooms that meet Biosafety Level 2 (BSL-2) standards, with designated areas for working with viruses and viral vectors. Additionally, we provide access to a broad collection of cell lines. 

Services:

• Culturing primary and established cell lines
• Derivation of induced pluripotent stem cells from clinical material
• Differentiation and reprogramming of stem cells
• Morphology, electrophysiology, proliferation, adhesion, migration, and cell viability analysis
• Cytotoxicity testing
• Mycoplasma testing
• Biobanking of cell lines
• Thermal sterilization of culture media, buffers, glassware, and laboratory materials
• Support in preparing research funding applications involving in vitro models

Cell culture

• 9 independent workstations meeting BSL-2 standards (fully equipped laminar flow cabinets by Alchem, Holten, Alpina), including 4 stations for work with viruses
• CO2 and CO2/O2 incubators (Memmert, Thermo Scientific, Binder)
• CO2-free incubators dedicated to insect cell cultures
• Orbital shakers for suspension cell culture

Preparation of biological material

• Lonza 4D-Nucleofector X-Unit electroporation system
• Low- and high-speed table centrifuges by Eppendorf (5702R, 5810R)
• Beckman Coulter Optima XPN-80, XPN-100 ultracentrifuges (rotors: TY-70.1Ti, SW-32.1TI)
• Beckman Coulter Avanti JXN-26 high-speed centrifuge (rotor: JA-30.50)
• Thermo Scientific CryoStar NX70 cryostat

Cell and nanoparticle analysis

• Automatic cell counters: Bio-Rad TC20, Invitrogen Countess 3 FL (RFP/GFP/DAPI/Cy5)
• Optical microscopes with phase contrast: Nikon Eclipse Ts2 (with camera), Leica DM IL LED
• Leica DM IL LED FL fluorescence microscope (GFP, 488 nm)
• Nikon Ti 2e inverted microscope with Crest X-Light confocal system, 7 lasers from 375 to 750 nm, modules for STED, FLIM, TIRF, electrophysiology (patch-clamp) analysis, and micro-patterning (Alveole PRIMO)
• Malvern Panalytical NS300 nanoparticle analyser (488 nm, 532 nm)

Biobanking

• Dewars for long-term sample storage in liquid nitrogen, -80°C freezers

Sterilization

• Laboratory washers, dryers, and steam autoclaves

• ECBG – European Center for Bioinformatics and Genomics – MOSAIC 3D (FENG.02.04-IP.04-0012/24). The project is co-funded through European Funds for Smart Economy 2021-2027 Programme (FENG), Action 2.4 Smart Economy Research Infrastructure. Project duration: 2025-2028.
  
  
• INTERCEPT – Targeted single-cell technology for cancer diagnostics – towards cell-oriented interceptive medicine (UoF/02-WIB-3/2023-004). The project is funded under the Virtual Research Institute Programme from the Polish Science Fund. Project duration: 2024-2028.
  
  
• Algorithm for the personalizing ovarian cancer treatment based on a spatial transcriptomic model of tumor tissue with single-cell resolution (FENG.02.02-IP.05-0361/23). FIRST TEAM FNP. Project co-financed by the European Regional Development Fund under the European Funds for a Modern Economy 2021–2027 (FENG) program. Project duration: 2024-2028.
  
  
• Phenotypic characteristics of cardiac organoids as a model of arrhythmogenic right ventricular cardiomyopathy obtained from patient material (2024/08/X/NZ3/01338). National Science Center MINIATURA 8. Project duration: 2024-2025.
  
  
• In addition, the Laboratory’s infrastructure is used by IBCH PAS teams to carry out over 25 projects funded by the National Science Centre (NCN), the National Centre for Research and Development (NCBiR), the Medical Research Agency (ABM), and Horizon Europe.