Cell and Tissue Culture Laboratory

Laboratory of Cell and Tissue Culture

Dr. Natalia Koralewska

Head of Laboratory

nataliak@man.poznan.pl
ext. 1505

The Laboratory is a platform that provides specialized equipment and conditions (sterility, controlled temperature, humidity etc.) for conducting (highly complex) experiments in the scope of a broadly defined area of molecular biology with the use of cultures of cell lines and tissues.
The Laboratory’s infrastructure and equipment ensures safe work (requirements of the BSL2 biosafety regulations) with animal as well as plant origin material. Self-contained areas for experiments involving virus and planaria type material are also available. All laboratory premises and equipment are subject to restrictive sterility rules (UV sterilization, ozonation), and waste collecting (Vacusafe-Integra) security systems.
In the Laboratory, once a special permit is obtained, work with transgenic material is also allowed.
The character of the Laboratory allows performing full-cycle experiments, from the propagation of the biological material through putting it to use in experiments, up to obtaining material for further analyses outside the CTCL block.
The CTCL provides services (for a fee) which include realization of external projects and services within the scope of experimental biology.

laminar flow cabinets (Holten, Alpina)
incubators with controlled carbon dioxide atmosphere (Memmert)
incubators with controlled oxygen concentration atmosphere (Thermo Scientific)
ultra-, preparative-, mini- and table-centrifuges (Beckman/Eppendorf)
rotary incubators (New Brunschwick, Innowa)
cooled incubators (Binder)
water baths
sample storage tanks with liquid nitrogen atmosphere
biolistic particle delivery system (BioRad PDS-1000/He)
microinjector Nanoject III (Drummond).
controlled environment tissue culture rooms, plant growth chambers (Persival) and computer controlled phytotron cluster
microscopic equipment fitted with specialized analysis and documentation software

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inverted microscopes (Leica DM IL LED with fluorescence and CoolLED illumination system, Leica DM500)
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stereoscopic microscopes (Leica M205 FA, Nikon SMZ1270, Nikon SMZ800N, Nikon SMZ-10, Motic SMZ-171)